Particle Cell Interactions
Particle Cell Interactions - Related Videos
13:15
Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
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Cited by 3 •
2014
Colloidal probe nanoscopy can be used within a variety of fields to gain insight into the physical stability and coagulation kinetics of colloidal systems and aid in drug discovery and formulation sciences using biological systems. The method described within provides a quantitative and qualitative means to study such systems.
08:11
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
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Cited by 7 •
2022
Minimizing the variability in the particle fraction within granular scaffolds facilitates reproducible experimentation. This work describes methods for generating granular scaffolds with controlled particle fractions for in vitro tissue engineering applications.
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
06:41
Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks
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Cited by 1 •
2024
An advanced particle selection method for cryo-EM, namely CryoSieve, improves density map resolution by removing a majority of particles in final stacks, as demonstrated through its application on a real-world dataset.
07:06
Particle Agglutination Method for Poliovirus Identification
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2011
A recently developed novel particle agglutination (PA) assay utilizing virus receptor molecule allowed a rapid and easy identification of poliovirus (PV). In this article, we will show the procedure for the PA assay for PV identification.
FAQs
Proteins from surrounding biological fluids adsorb onto particle surfaces and can alter how cells recognize, bind, and internalize them. This protein layer links the particle’s original surface chemistry and charge to subsequent receptor interactions, endocytosis, or phagocytosis. Accounting for this behavior is therefore important when designing bioengineered particles intended to interact predictably with cells.
Size, shape, surface chemistry, and charge can each affect membrane binding, entry, intracellular trafficking, and cellular responses. These properties do not act independently because adsorbed proteins may further modify the particle surface in biological fluids. Controlling and comparing these features helps bioengineers relate particle design to uptake efficiency, cell viability, signaling, inflammation, and toxicity.
Endocytosis and phagocytosis represent distinct routes by which cells internalize particles. Receptor-mediated endocytosis links entry to specific cellular recognition, whereas phagocytosis supports particle uptake by cells capable of engulfing larger or recognized material. The route selected influences intracellular trafficking and the resulting cellular effects, making uptake mechanism relevant to delivery design and safety assessment.
Entry alone does not determine a particle’s biological effect. Intracellular trafficking governs where the particle moves after internalization and can influence whether it supports delivery, alters signaling, or contributes to toxicity. Examining this stage helps researchers connect particle properties and uptake pathways with outcomes such as cell viability, inflammation, and intended bioengineering performance.
A useful evaluation considers particle size, shape, surface chemistry, charge, and the proteins adsorbed from biological fluids. Researchers should also examine membrane binding, internalization through endocytosis or phagocytosis, intracellular trafficking, cell viability, signaling, inflammation, and potential toxicity. Together, these observations connect material characteristics with cellular and tissue-level responses.
These interactions guide the design of particles for targeted drug and gene delivery, diagnostic imaging, and biosensing. They also inform tissue-engineering strategies by showing how materials influence cells. In parallel, studying viability, signaling, inflammation, toxicity, and tissue-level responses supports safer material development and helps determine whether a particle produces the intended biological outcome.