K-fold Cross-validation

K-fold cross-validation is a resampling method for evaluating how well a statistical or machine-learning model will perform on unseen data. It partitions a dataset into k complementary subsets, or folds, trains the model on k−1 folds, and tests it on the remaining fold; repeating this process until each fold serves as the validation set produces an average performance estimate. In bioengineering, this approach helps assess models built from biological measurements, medical images, or biomaterial data while reducing dependence on a single train-test split. It also supports model comparison, hyperparameter selection, and detection of overfitting when datasets are limited.

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JoVE Core - Electrical Engineering

Reclosers and Fuses

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2024

Automatic circuit reclosers enhance the protection of distribution circuits by interrupting and auto-reclosing an AC circuit according to a preset sequence. They effectively manage temporary faults on overhead distribution lines, often caused by tree limbs or wildlife, by briefly disrupting service to improve overall reliability. However, contact with reclosers or energized broken conductors on the ground can pose serious hazards. A comprehensive protection scheme for radial distribution...

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2023

Source: Laboratory of Alan Lester - University of Colorado Boulder Geologic maps were first made and utilized in Europe, in the mid-to-late 18th century. Ever since, they have been an important part of geological investigations all around the world that strive to understand rock distributions on the surface of the earth, in the subsurface, and their modification through time. A modern geologic map is a data-rich representation of rocks and rock-structures in a two-dimensional plan view. The...

Molecular Chaperones and Protein Folding

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2020

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein. The...

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JoVE Journal - Bioengineering
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Microfluidic Mixers for Studying Protein Folding

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Cited by 9 •

2012

In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).

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2022

Here, we describe protocols for the preparation of trans-cyclobutane fused cyclooctenes (tCBCO), their polymerization to prepare depolymerizable olefinic polymers, and the depolymerization of these polymers under mild conditions. Additionally, protocols for the preparation of depolymerizable networks and compression molding of rigid linear plastics based on this system are described.

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