Endothelial Medium Perfusion
Endothelial Medium Perfusion - Related Videos
08:54
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
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Cited by 75 •
2018
The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.
10:18
Isolated Lung Perfusion System in the Rabbit Model
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2021
The isolated rabbit lung preparation is a gold standard tool in pulmonary research. This publication aims to describe the technique as developed for the study of physiological and pathological mechanisms involved in airway reactivity, lung preservation, and preclinical research in lung transplantation and pulmonary edema.
05:41
Non-invasive Assessment of Microvascular and Endothelial Function
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Cited by 12 •
2013
Capillaroscopy is a non-invasive, relatively inexpensive methodology for directly visualizing the microcirculation. The forearm blood flow technique provides accepted non-invasive measures of endothelial function.
08:20
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Cited by 75 •
2016
The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
11:04
Isolation of Valvular Endothelial Cells
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Cited by 82 •
2010
We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
FAQs
Fluid shear stress acts as a mechanical cue rather than simply a transport condition. As medium moves across or through an endothelial construct, the resulting force can influence cell alignment, barrier function, and vascular signaling. Controlling this exposure lets investigators examine how endothelial organization and function change under defined flow conditions.
The pump or fluidic system sets the circulation that carries medium through the experimental construct. Its control over flow determines how consistently cells receive nutrients and oxygen and how predictably they experience shear stress. This controlled delivery is important when comparing endothelial responses, because changes in flow can be linked to differences in alignment, barrier behavior, or vascular signaling.
Compared with static culture, perfusion adds a continuously controlled flow environment rather than leaving medium stationary around the cells. That difference provides ongoing nutrient and oxygen delivery and a mechanical stimulus from shear stress. Consequently, engineered tissues, blood vessel models, and organ-on-chip systems can be studied under conditions intended to better represent vascular environments.
A setup begins with an endothelial cell construct positioned so medium can pass over or through it. The construct is connected to a pump or fluidic path, and circulation is adjusted to provide nutrient and oxygen delivery while applying flow. Researchers then assess responses such as alignment, barrier function, vascular signaling, or permeability.
The method supports in vitro blood vessel models, organ-on-chip systems, and engineered tissues by introducing flow into endothelial culture. These platforms can be used to investigate vascular disease, drug transport, permeability, and endothelial responses to controlled changes in flow. The shared advantage is the ability to connect a defined fluid environment with measurable vascular behavior.
Researchers can relate changes in alignment, barrier function, vascular signaling, or permeability to the flow conditions imposed by the system. They can also examine how controlled flow affects drug transport or responses relevant to vascular disease. This interpretation links the physical environment created by perfusion with functional endothelial outcomes in engineered models.