Large Volume Illuminator
Large Volume Illuminator - Related Videos
08:32
Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates
0 Views •
Cited by 5 •
2017
A protocol to build a tissue penetrating illuminator for delivering light over large volumes with minimal diameter is presented.
03:22
Phototaxis Assay of Filamentous Cyanobacteria Using Focused Light Illumination
0 Views •
2025
Source: Weber, N. et. al., Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna. J. Vis. Exp. (2022)This video demonstrates a focused LED-based assay for studying phototaxis in filamentous cyanobacteria. The bacteria migrate toward the light source, forming a dense accumulation at the illuminated center, which is visualized and quantified through pixel intensity analysis of the captured image.
06:14
Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
0 Views •
Cited by 1 •
2022
The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.
10:07
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
0 Views •
2014
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
06:16
Monitoring the Effects of Illumination on the Structure of Conjugated Polymer Gels Using Neutron Scattering
0 Views •
Cited by 3 •
2017
A protocol for the analysis of gels formed from the optoelectronic conjugated polymer poly(3-hexylthiophene-2,5-diyl) (P3HT) using small and ultra-small angle neutron scattering in both the presence and absence of illumination is presented.
FAQs
The key advantage is continuity of observation as a subject moves through three-dimensional space. A small focal plane can limit visibility to one localized region, whereas distributed excitation keeps more of the experimental volume available for imaging. This broader coverage helps relate movement to position and reduces the chance that behavior is missed when the subject leaves a narrow viewing zone.
Controlled illumination must be coordinated with video acquisition so the illuminated region matches the camera’s behavioral field. When these areas align, freely moving organisms can remain visible during recording rather than appearing only when they enter a restricted focal area. This improves the continuity of behavioral measurements across the experiment.
Controlled illumination makes the subject’s location part of the behavioral observation rather than treating movement as an isolated event within one viewing plane. By maintaining visibility across an extended region, the approach helps investigators examine how movement unfolds in relation to the surrounding experimental space and the conditions encountered during recording.
A Large Volume Illuminator is especially relevant when subjects move freely and may travel beyond a narrowly defined imaging region. Restricting illumination to a small focal plane can interrupt observation as the subject changes position. Extending the observable region supports behavioral recording across larger spaces, making it more suitable for experiments centered on continuous movement.
The illumination should cover the region in which the subject is expected to move, while the video acquisition system should capture that same region. Researchers can then observe the subject throughout the relevant experimental space instead of coordinating separate observations across disconnected areas. This spatial match supports consistent recording of behavior as conditions or positions change.
By keeping moving subjects visible across a larger region, the method supports analysis of movement, posture, interactions, and responses to environmental or experimental stimuli. Its value extends beyond detecting whether a subject is present: the expanded field helps connect those behaviors with spatial context and with the conditions experienced during the experiment.