Multilevel Microdissection and Functional-Structural Profiling of Human Renal Arterial Branches

348 просмотров

06:51 мин.

5 сентября 2025 г.

10.3791/68579-v

5 сентября 2025 г.

348 просмотров

Xuya Kang¹ , Yingjia Li¹ , Junxia Zhang¹^,²^,³ , Xinying Wang¹ , Lin Yao⁴ , Yan Zhang¹^,²^,³^,⁵^,⁶ , Yahan Liu¹

¹Institute of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University Health Science Center; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, ²Beijing Key Laboratory of Cardiovascular Receptors Research, ³Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, Peking University, ⁴Department of Urology, Peking University First Hospital; Institute of Urology, Peking University; Beijing Key Laboratory of Urogenital Diseases (Male) Molecular Diagnosis and Treatment Center, National Urological Cancer Center, ⁵Institute of Cardiovascular Diseases, First Affiliated Hospital of Dalian Medical University, ⁶NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Peking University

This article presents a step-by-step protocol for the isolation and functional evaluation of human renal arterial branches, facilitating preclinical studies for pharmaceutical development.

Больше видео

Microdissection Technique

Wire Myography

Vascular Dysfunction

Intrarenal Artery Isolation

Vasomotor Responses

Muscle Length-Tension

Hematoxylin Eosin Staining

Phenylephrine Contraction

Arterial Normalization

Chapters in this video