Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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Цитируется: 8

06:43 мин.

3 мая 2022 г.

10.3791/63730-v

3 мая 2022 г.

1.1K просмотров

Yazgan Tuna¹ , Amer Al-Hiyasat¹ , Jonathon Howard¹^,²

¹Department of Molecular Biophysics & Biochemistry, Yale University, ²Department of Physics, Yale University

We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

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Interference Reflection Microscopy

TIRF Microscopy

Microtubule Imaging

Microtubule Associated Proteins

Label-Free Imaging

Kinesin Motility

Fluorescent Microbeads

Image Registration

High Resolution Imaging

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