Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
2.1K просмотров
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Цитируется: 2
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12:26 мин.
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29 января 2022 г.
DOI :10.3791/63121-v
29 января 2022 г.
•2.1K просмотров
Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.
Chapters in this video
0:04
Introduction
1:22
Preparation of Methacrylated Coverslips
2:39
Micropatterning of Extracellular Matrix Proteins on Glass Coverslips
5:31
Fabrication of Patterned Polyacrylamide Hydrogels
9:15
Local Release of Cell Traction Forces by UV-A Laser Illumination
10:26
Image Processing, Particle Image Velocity and Calculation of Traction Forces
10:55
Results
11:56
Conclusion
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