Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

907 просмотров

Цитируется: 4

07:19 мин.

5 ноября 2018 г.

10.3791/58538-v

5 ноября 2018 г.

907 просмотров

Tyler Mrozowich¹ , Steffane McLennan² , Michael Overduin²^,⁴ , Trushar R. Patel¹^,³^,⁴

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, ²Department of Biochemistry, University of Alberta, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

Here, we present how Small Angle X-Ray Scattering (SAXS) can be utilized to obtain information on low-resolution envelopes representing the macromolecular structures. When used in conjunction with high-resolution structural techniques such as X-Ray Crystallography and Nuclear Magnetic Resonance, SAXS can provide detailed insights into multidomain proteins and macromolecular complexes in-solution.