Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

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Цитируется: 10

08:26 мин.

14 июня 2018 г.

10.3791/57421-v

14 июня 2018 г.

720 просмотров

Alba Llibre^*¹^,² , Vincent Bondet^*¹^,² , Mathieu P. Rodero³ , David Hunt⁴ , Yanick J. Crow³^,⁵ , Darragh Duffy¹^,²

¹Immunobiology of Dendritic Cells, Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, ⁵Manchester Centre for Genomic Medicine, University of Manchester

^* These authors contributed equally

Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

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Digital ELISA

Interferon Alpha Detection

Specificity Validation

Reproducibility Assessment

Cytokine Quantification

Chapters in this video