Concurrent Quantification of Cellular and Extracellular Components of Biofilms

346 просмотров

Цитируется: 18

10:18 мин.

10 декабря 2013 г.

10.3791/50639-v

10 декабря 2013 г.

346 просмотров

Sharukh S. Khajotia¹ , Kristin H. Smart¹ , Mpala Pilula¹^,³ , David M. Thompson²

¹Department of Dental Materials, College of Dentistry, University of Oklahoma Health Sciences Center, ²Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, ³Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.