Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination

892 просмотров

Цитируется: 9

07:42 мин.

1 августа 2011 г.

10.3791/2823-v

1 августа 2011 г.

892 просмотров

Daniel A. Gibson¹^,² , Le Ma²^,³

¹Neuroscience Graduate Program, Keck School of Medicine, University of Southern California, ²Zilkha Neurogenetic Institute, University of Southern California, ³Department of Cell and Neurobiology, Neuroscience Graduate Program, Keck School of Medicine, University of Southern California

An in vivo method to test gene function in postnatal brain is described. Recombinant AAVs expressing Cre and/or a fluorescent protein are injected into neonatal mouse brain. Mosaic gene inactivation and sparse neuronal labeling are achieved, allowing rapid analysis of gene function in processes critical to neural circuit development.