Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

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Цитируется: 2

10:36 мин.

21 декабря 2010 г.

10.3791/2220-v

21 декабря 2010 г.

616 просмотров

H. Arthur Jeiranian^*¹ , Brandy J. Schalow^*¹ , Justin Courcelle¹

¹Department of Biology, Portland State University

^* These authors contributed equally

We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.

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Two-dimensional Gel Electrophoresis

DNA Replication Intermediates

UV-induced DNA Damage

Plasmid PBR322 Analysis

Southern Blotting

Restriction Enzyme Digestion

Alkali Transfer System

Replication Fork Reversal

Nucleotide Excision Repair

Chapters in this video