Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

1.4K просмотров

Цитируется: 69

10:19 мин.

10 августа 2010 г.

10 августа 2010 г.

1.4K просмотров

Alexandra Cretu¹ , Paola Castagnino¹ , Richard Assoian¹

¹Department of Pharmacology, University of Pennsylvania

The effect of substrata stiffness on cellular function can be modeled in vitro using polyacrylamide hydrogels of varying compliances.

Больше видео

Acrylamide Hydrogels

Extracellular Matrix

Reactive Cover Slips

Glutaraldehyde Cross-linking

Fibronectin Coating

Rheology Analysis

Atomic Force Microscopy

Chapters in this video

0:04

Title

0:49

Introduction

1:10

Coverslip Preparation

3:50

Preparation of Hydrogels

6:15

Modeling

8:35

Representative Results

9:50

Conclustion