Name: Captura de célula usando um dispositivo micro (Vídeo) | JoVE Journal
Uploaded: 2007-10-01T15:28:15.000+00:00
Duration: 29 min 2 s
Description: Aprenda a capturar células usando dispositivos microfluídicos para pesquisa em neurociência. Este método permite isolar neutrófilos e linfócitos CD4+ de sangue total com alta eficiência.

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cell capture using a microfluidic device

Cell Capture Using a Microfluidic Device

The devices as we Prepared them, are packaged into kits of 10 or 20, and when they arrive, they're shipped cold in a box and the kit contains all the components that you need to run. The experiment in the kit are, is a packet with all the syringes and buffers, which can remain at room temperature. There's also a copy of the protocol that we're gonna follow in this experiment.There's also a refrigerated box, which contains the actual devices and all the running buffers which need to be refrigerated. Typically, kits are packaged in groups of 10 or 20. When not in use, the kits should be refrigerated.Each syringe and each kit component is carefully labeled and the labels correspond to the table in the protocol that we follow. So there's no ambiguity as to which syringe or kit component should be used in the individual steps. After unpacking the, the devices and placing them into a biological safety cabinet, we'll be performing the procedure of isolating the neutrophils and lysing them for downstream genomic applications because we're gonna be, because the life save that we get will be used for isolating or, or we will extract the RNA from the cell lysate.Because of that, we wanna make

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Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

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Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

The clinical microfluidic chip is an important biomedical analysis technique that simplifies clinical patient blood sample preprocessing and immunofluorescently stains circulating tumor cells (CTCs) in situ on the chip, allowing the rapid detection and identification of a single CTC.

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

The protocol describes an advanced microfluidic platform to quantitatively measure cytokine secretion dynamics of individual human peripheral blood mononuclear cells. The platform measures up to three cytokines in parallel (IL-6, TNFα, and IL-1β) for each individual cell stimulated with lipopolysaccharide as an example.

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Aprenda a capturar células usando dispositivos microfluídicos para pesquisa em neurociência. Este método permite isolar neutrófilos e linfócitos CD4+ de sangue total com alta eficiência.

Cell Capture Using a Microfluidic Device

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