Name: マイクロ流体デバイスを用いた細胞のキャプチャ (動画) | JoVE Journal
Uploaded: 2007-10-01T15:28:15.000+00:00
Duration: 29 min 2 s
Description: 学ぶマイクロ流体デバイスを用いた細胞キャプチャと免疫蛍光染色法。全血からCD4陽性リンパ球を分離し、抗体染色とDAPI核染色で細胞を可視化する技術を解説します。

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cell capture using a microfluidic device

Cell Capture Using a Microfluidic Device

The devices as we Prepared them, are packaged into kits of 10 or 20, and when they arrive, they're shipped cold in a box and the kit contains all the components that you need to run. The experiment in the kit are, is a packet with all the syringes and buffers, which can remain at room temperature. There's also a copy of the protocol that we're gonna follow in this experiment.There's also a refrigerated box, which contains the actual devices and all the running buffers which need to be refrigerated. Typically, kits are packaged in groups of 10 or 20. When not in use, the kits should be refrigerated.Each syringe and each kit component is carefully labeled and the labels correspond to the table in the protocol that we follow. So there's no ambiguity as to which syringe or kit component should be used in the individual steps. After unpacking the, the devices and placing them into a biological safety cabinet, we'll be performing the procedure of isolating the neutrophils and lysing them for downstream genomic applications because we're gonna be, because the life save that we get will be used for isolating or, or we will extract the RNA from the cell lysate.Because of that, we wanna make

Research

JoVE Journal

Biology

We describe a protocol for the fabrication of microfluidic devices that can enable cell capture and culture.  In this approach patterned microstructures such as grooves within microfluidic channels are used to create low shear stress regions within which cell can dock.

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

In this article, we present a microfluidic-based method for particle confinement based on hydrodynamic flow.  We demonstrate stable particle trapping at a fluid stagnation point using a feedback control mechanism, thereby enabling confinement and micromanipulation of arbitrary particles in an integrated microdevice.

A Microfluidic-based Hydrodynamic Trap for Single Particles

In this article we present a microfluidic chip for single cell analysis. It allows the quantification of intracellular proteins, enzymes, cofactors, and second messengers by means of fluorescent assays or...

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This protocol provides detailed steps on how to use the system for a broad range of applications.

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Here, we demonstrate a simple production method for size-controllable, monodisperse, water-in-oil (W/O) microdroplets using a capillary-based centrifugal microfluidic device. This method requires only a small sample volume and enables high-yield production. We expect this method will be useful for rapid biochemical and cellular analyses.

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

The clinical microfluidic chip is an important biomedical analysis technique that simplifies clinical patient blood sample preprocessing and immunofluorescently stains circulating tumor cells (CTCs) in situ on the chip, allowing the rapid detection and identification of a single CTC.

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

The protocol describes an advanced microfluidic platform to quantitatively measure cytokine secretion dynamics of individual human peripheral blood mononuclear cells. The platform measures up to three cytokines in parallel (IL-6, TNFα, and IL-1β) for each individual cell stimulated with lipopolysaccharide as an example.

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

学ぶマイクロ流体デバイスを用いた細胞キャプチャと免疫蛍光染色法。全血からCD4陽性リンパ球を分離し、抗体染色とDAPI核染色で細胞を可視化する技術を解説します。

Cell Capture Using a Microfluidic Device

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