Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

2.1K צפיות

צוטט 2 פעמים

12:26 דק׳

29 בינואר 2022

10.3791/63121-v

29 בינואר 2022

2.1K צפיות

Joel Christian¹ , Johannes W. Blumberg² , Dimitri Probst² , Cristina Lo Giudice¹ , Sandra Sindt³ , Christine Selhuber-Unkel³ , Ulrich S. Schwarz² , Elisabetta Ada Cavalcanti-Adam¹

¹Department of Cellular Biophysics, Max Planck Institute for Medical Research, ²Institute for Theoretical Physics and BioQuant, Heidelberg University, ³Institute for Molecular Systems Engineering (IMSE), Heidelberg University

Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

גלה סרטונים נוספים

Polyacrylamide Hydrogels

Cell Adhesion Control

Micropatterned Hydrogels

Fluorescence Microscopy

UV Photolithography

Extracellular Matrix Proteins

Fourier Transform Traction Cytometry

Atomic Force Microscopy

Chapters in this video