Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

663 צפיות

צוטט 8 פעמים

12:15 דק׳

9 באפריל 2019

10.3791/59314-v

9 באפריל 2019

663 צפיות

Carlos Oscar S. Sorzano^*¹^,² , Laura Martínez-Muñoz^*³^,⁴ , Graciela Cascio³^,⁵ , Eva M. García-Cuesta³ , J. Vargas¹^,⁶ , Mario Mellado³ , Jose Miguel Rodriguez Frade³

¹Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, ²Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, ³Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, ⁴Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), ⁵Meyer Cancer Center, ⁶Department of Anatomy and Cell Biology, McGill Univ.

^* These authors contributed equally

Here, we present a protocol for single particle tracking image analysis that allows quantitative evaluation of diffusion coefficients, types of motion and cluster sizes of single particles detected by fluorescence microscopy.

גלה סרטונים נוספים

Single Molecule Tracking

Particle Tracking

Diffusion Analysis

Cluster Size Analysis

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