Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

743 vues

Cité par 12

00:07 min

21 août 2019

10.3791/60029-v

21 août 2019

743 vues

Emily A. Brown¹^,² , Steven C. Neier³^,⁴ , Claudia Neuhauser⁵ , Adam G. Schrum⁶^,⁷^,⁸ , Stephen E.P. Smith¹^,²^,⁹

¹Center for Integrative Brain Research, Seattle Children's Research Institute, ²Graduate Program in Neuroscience, University of Washington, ³Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, ⁴Broad Institute of Harvard and MIT, ⁵Department of Mathematics, University of Houston, ⁶Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, ⁷Department of Surgery, School of Medicine, University of Missouri, ⁸Department Bioengineering, College of Engineering, University of Missouri, ⁹Department of Pediatrics, University of Washington

Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.