Name: Captura de células utilizando un dispositivo de microfluidos (Video) | JoVE Journal
Uploaded: 2007-10-01T15:28:15.000+00:00
Duration: 29 min 2 s
Description: Aprende a capturar células sanguíneas utilizando un dispositivo de microfluidos para aislamiento de neutrófilos y linfocitos CD4. Este método permite el análisis genómico y la tinción inmunofluorescente directamente en el dispositivo.

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cell capture using a microfluidic device

Cell Capture Using a Microfluidic Device

The devices as we Prepared them, are packaged into kits of 10 or 20, and when they arrive, they're shipped cold in a box and the kit contains all the components that you need to run. The experiment in the kit are, is a packet with all the syringes and buffers, which can remain at room temperature. There's also a copy of the protocol that we're gonna follow in this experiment.There's also a refrigerated box, which contains the actual devices and all the running buffers which need to be refrigerated. Typically, kits are packaged in groups of 10 or 20. When not in use, the kits should be refrigerated.Each syringe and each kit component is carefully labeled and the labels correspond to the table in the protocol that we follow. So there's no ambiguity as to which syringe or kit component should be used in the individual steps. After unpacking the, the devices and placing them into a biological safety cabinet, we'll be performing the procedure of isolating the neutrophils and lysing them for downstream genomic applications because we're gonna be, because the life save that we get will be used for isolating or, or we will extract the RNA from the cell lysate.Because of that, we wanna make

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Aprende a capturar células sanguíneas utilizando un dispositivo de microfluidos para aislamiento de neutrófilos y linfocitos CD4. Este método permite el análisis genómico y la tinción inmunofluorescente directamente en el dispositivo.

Cell Capture Using a Microfluidic Device

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