Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

424 مشاهدة

استشهد بها 1

04:30 دقيقة

يناير 17, 2025

10.3791/67331-v

يناير 17, 2025

424 مشاهدة

Francisco Javier Álvarez-Martínez¹ , María Herranz-López¹ , Enrique Barrajón-Catalán¹ , Vicente Micol¹^,²

¹Institute for Research, Development and Innovation in Health Biotechnology of Elche (IDiBE), Miguel Hernández University (UMH), ²CIBER, Pathophysiology of Obesity and Nutrition, CIBERobn, Carlos III Health Institute (CB12/03/30038)

This protocol describes the development of a colorimetric assay method for determining the ability of compounds to inhibit or activate elastase activity.

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Absorbance Measurement

Reaction Buffer

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Introduction

0:58

Elastase Activity Modulation by Phytochemicals: A Colorimetric Assay-Based Approach for Drug Development and Research