Miniscope Recording Calcium Signals at Hippocampus of Mice Navigating an Odor Plume

327 مشاهدة

استشهد بها 2

06:21 دقيقة

سبتمبر 20, 2024

10.3791/67039-v

سبتمبر 20, 2024

327 مشاهدة

Fabio M. Simoes de Souza¹ , Ryan Williamson² , Connor McCullough³ , Alec Teel¹ , Gregory Futia³ , Ming Ma¹ , Aaron True⁴ , John P. Crimaldi⁴ , Emily Gibson³^,⁵ , Diego Restrepo¹^,⁵

¹Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, ²Neurotechnology Center, University of Colorado Anschutz Medical Campus, ³Department of Bioengineering, University of Colorado Anschutz Medical Campus, ⁴Civil, Environmental & Architectural Engineering, University of Colorado, ⁵Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus

This protocol investigates the brain-behavior relationship in hippocampal CA1 in mice navigating an odor plume. We provide a step-by-step protocol, including surgery to access imaging of the hippocampus, behavioral training, miniscope GCaMP6f recording and processing of the brain, and behavioral data to decode the mouse position from ROI neural activity.

استكشف المزيد من الفيديوهات

Calcium Imaging

Hippocampus CA1

Odor Plume Navigation

GCaMP6f Mice

GRIN Lens Imaging

Motion Correction

Neural Ensemble Activity

Photoionization Detector

Spatial Trajectory Decoding

Chapters in this video

0:00

Introduction

1:11

Setting up the Odor Arena and Photoionization Detector Sensors for Mouse Odor Plume Navigation Studies

3:04

Behavioral Training and Epifluorescence Recording of a Freely Moving Mouse in the Odor Arena for Monitoring CA1 Neuronal Activity to Decode Spatial Navigation