High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

456 مشاهدة

استشهد بها 7

10:51 دقيقة

ديسمبر 5, 2016

10.3791/54831-v

ديسمبر 5, 2016

456 مشاهدة

Michal Breker¹ , Kristi Lieberman¹ , Frej Tulin² , Frederick R. Cross¹

¹Laboratory of Cell Cycle Genetics, The Rockefeller University, ²Sainsbury Laboratory, University of Cambridge

Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.

استكشف المزيد من الفيديوهات

Temperature-sensitive Mutants

Robotics-based Pipeline

Complementation Testing

Linkage Testing

Gametogenesis Assay

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