Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

295 مشاهدة

استشهد بها 19

08:11 دقيقة

مارس 31, 2013

10.3791/50038-v

مارس 31, 2013

295 مشاهدة

Guangliang Wang¹ , H. Joseph Yost² , Jeffrey D. Amack¹

¹Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, ²Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics, University of Utah

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.