A Protocol for Computer-Based Protein Structure and Function Prediction

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استشهد بها 79

16:41 دقيقة

نوفمبر 3, 2011

10.3791/3259-v

نوفمبر 3, 2011

4.3K مشاهدة

Ambrish Roy¹^,² , Dong Xu¹ , Jonathan Poisson¹ , Yang Zhang¹^,²

¹Center for Computational Medicine and Bioinformatics, University of Michigan , ²Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.