Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

616 مشاهدة

استشهد بها 2

10:36 دقيقة

ديسمبر 21, 2010

10.3791/2220-v

ديسمبر 21, 2010

616 مشاهدة

H. Arthur Jeiranian^*¹ , Brandy J. Schalow^*¹ , Justin Courcelle¹

¹Department of Biology, Portland State University

^* These authors contributed equally

We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.

استكشف المزيد من الفيديوهات

Two-dimensional Gel Electrophoresis

DNA Replication Intermediates

UV-induced DNA Damage

Plasmid PBR322 Analysis

Southern Blotting

Restriction Enzyme Digestion

Alkali Transfer System

Replication Fork Reversal

Nucleotide Excision Repair

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