Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
مارس 24, 2010
637 مشاهدة
Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.